anti b7 h3 antibody  (R&D Systems)

Journal: Molecular and Clinical Oncology

Article Title: A cascade via CD276/PI3K/SIRT1/E-Cad in overcoming contact inhibition of proliferation in hepatocellular carcinoma cells

doi: 10.3892/mco.2026.2941

Figure Lengend Snippet: SIRT1 acts as a downstream regulator of CD276 in mediating E-Cad. (A) The expression of SIRT1 was analyzed by western blotting in HepLi5, SNU-449 and 3B cell lines under sparse or confluent conditions. (B) The expression of SIRT1 in 449-OX cells was analyzed by western blotting under three conditions: sparse, confluent, and confluent with additional treatment of LY294002. (C) The expression of SIRT1 was analyzed by western blotting in 3B-KD cells under sparse and confluent conditions. 3B, Hep 3B2.1-7; 449-OX, CD276-ovexpressing SNU-449 cells; Li5, HepLi5; 449, SNU-449; 3B, Hep 3B2.1-7; S, sparse cultures; C, confluent cultures; LY, LY294002; si-NC, small interfering-negative control; 3B-KD, CD276-knockdown Hep 3B2.1-7 cells.

Article Snippet: Membranes were then blocked with 5% non-fat milk for 1 h at room temperature and incubated overnight at 4 ̊C with primary antibodies: CD276 (1:1,000; cat. no. A17216), E-Cad (1:2,000; cat. no. A24874), p27 (1:1,000; cat. no. A0290), cyclin D1 (1:1,000; cat. no. A11310), GAPDH (1:50,000; cat. no. A19056) and SIRT1 (1:2,000; cat. no. A0230; all from ABclonal Biotech Co., Ltd.).

Techniques: Expressing, Western Blot, Negative Control, Knockdown

Journal: bioRxiv

Article Title: Transcription Factor–Mediated Reprogramming of Cancer-Associated Fibroblasts Reveals Targetable Vulnerabilities in Solid Tumors

doi: 10.64898/2026.04.15.718753

Figure Lengend Snippet: A. Immunocytochemistry (ICC) analysis demonstrates strong B7-H3 (CD276) expression on both PC-3 cancer cells and pCAFs, visualized by red fluorescence. B. In 2D co-culture assays, anti-B7-H3 CAR T cells exhibit antigen-specific cytotoxicity against PC-3 cells, with killing efficiency increasing across graded effector-to-target (E:T) ratios.

Article Snippet: Primary human anti–B7-H3 antibody (AF1027, R&D Systems) was diluted in cold staining buffer (0.5 μg/μL; 2 μL per 100 μL reaction volume) and applied to cells for 20–30 min at 4 °C.

Techniques: Immunocytochemistry, Expressing, Fluorescence, Co-Culture Assay

Journal: bioRxiv

Article Title: Transcription Factor–Mediated Reprogramming of Cancer-Associated Fibroblasts Reveals Targetable Vulnerabilities in Solid Tumors

doi: 10.64898/2026.04.15.718753

Figure Lengend Snippet: Stromal Effects on CAR T-Cell Activity in 3D-Bioprinted PC-3 Spheroids. A. Representative images of stromal populations influencing anti-B7-H3 CAR T-cell killing function . B. PC-3 spheroid with pCAF/rpNFs interaction with CAR-T cells monitored by IncuCyte imaging system. C. The killing effects of B7-H3 CAR T cells on PC-3 cells in day 5 compared to day 1. D . Imaging of live cells at different time.

Article Snippet: Primary human anti–B7-H3 antibody (AF1027, R&D Systems) was diluted in cold staining buffer (0.5 μg/μL; 2 μL per 100 μL reaction volume) and applied to cells for 20–30 min at 4 °C.

Techniques: Activity Assay, Imaging